Grant Funded Research Intern

Period: May, 2023 - August, 2023

Performed Kidney/Bladder/UTI research in the department of pediatrics and the department of nephrology through the SRIP (Student Research Internship Program) provided by UPMC Children’s Hospital of Pittsburgh, University of Pittsburgh, and a PHS Grant.

    ❖ Did small animal (mouse) work including gowning, sample collection, and asphyxiation.
    ❖ Did sample prep for imaging:
        ❖ Paraffinization → Microtomy → Staining → Imaging
        ❖ Staining includes H&E and IHC
    ❖ Developed a rudimentary cell counting neural network for IHC stains in my free time.

Award: Awarded a travel grant to ASN Kidney Week that totaled $3000 as a result of my research in addition to membership in the American Society of Nephrology. Provided by ASN and NIDDK.

SKILLS OBTAINED

Certifications (IDs on Resume):

    ❖ Working with Small Animals (05/23 - 05/27)
    ❖ Working with Mice in Research (05/23 - 05/27)
    ❖ Use of Controlled Substances in Basic & Animal Research (05/23 - 05/27)
    ❖ COI PHS Regulated Course (05/23 - 05/27)
    ❖ Responsible Conduct of Research (05/23 - 05/27)
    ❖ Bloodborne Pathogens for Lab Workers (05/23 - 05/24)

Lab Protocols:

    ❖ Sectioning (paraffin)
    ❖ IHC (Immunohistochemistry) stains
    ❖ H&E (Hematoxylin & Eosin) stains
    ❖ Fluorescence & Brightfield microscopy
    ❖ Technical writing & Poster presentation

Instruments:

    ❖ Microtome
    ❖ Incubator
    ❖ Leica DMi8 scope
    ❖ Confocal

Other Skills:

    ❖ Laboratory Safety
    ❖ Laboratory Etiquette

Project Sample