Extracellular Matrix Morphology and Cellular Mechanobiology on Interwoven v. Interwoven -Aligned PDMS Scaffolds

Objective: Observe variations in ECM morphology, nuclei morphometry, and differentiation due to topological cues on different tissue engineering scaffold patterns.

    ❖ Collagen I, fibronectin, and laminin stains were used to observe ECM morphology
    ❖ DAPI was used to analyze nuclei morphometry
    ❖ alpha-SMA and F-Actin (via phalloidin) stains were used to observe possible differentiation

SKILLS OBTAINED

Lab Protocols:

    ❖ Cell culture (hDF)
    ❖ IF stains
    ❖ PDMS casting
    ❖ RIE photolithography
    ❖ Technical writing & Poster presentation

Instruments:

    ❖ Zeiss Axio Observer 3
    ❖ Incubator
    ❖ Harrick Plasma Cleaner PDC-001
    ❖ Anprolene AN75i Ethylene Oxide Sterilizer

Other Skills:

    ❖ Mentoring and management
    ❖ GraphPad and Fiji ImageJ 

Project Sample