Chrm3-/- in mice modeling urothelial injury

Objective: Characterize urothelial injury in mice with knockout muscarinic acetylcholine receptor subtype M3 as a model for urothelial injury due to neurogenic bladder.

    ❖ H&E and IHC stains were used for morphological and protein expression characterization
    ❖ IHC utilized KRT5/14/20, UPK, and Ki67

SKILLS OBTAINED

Certifications (IDs on Resume):

    ❖ Working with Small Animals (05/23 - 05/27)
    ❖ Working with Mice in Research (05/23 - 05/27)
    ❖ Use of Controlled Substances in Basic & Animal Research (05/23 - 05/27)
    ❖ COI PHS Regulated Course (05/23 - 05/27)
    ❖ Responsible Conduct of Research (05/23 - 05/27)
    ❖ Bloodborne Pathogens for Lab Workers (05/23 - 05/24)

Lab Protocols:

    ❖ Sectioning (paraffin)
    ❖ IHC (Immunohistochemistry) stains
    ❖ H&E (Hematoxylin & Eosin) stains
    ❖ Fluorescence & Brightfield microscopy
    ❖ Technical writing & Poster presentation

Instruments:

    ❖ Microtome
    ❖ Incubator
    ❖ Leica DMi8 scope
    ❖ Confocal

Other Skills:

    ❖ Laboratory Safety
    ❖ Laboratory Etiquette

Project Sample